iLIF: illumination by Laser-Induced Fluorescence for single flash imaging on a nanoseconds timescale

The challenge in visualizing fast microscale fluid motion phenomena is to record high-quality images free of motion-blur. Here, we present an illumination technique based on laser-induced fluorescence which delivers high-intensity light pulses of 7 ns. The light source consists of a Q-switched Nd:YAG laser and a laser dye solution incorporated into a total internal reflection lens, resulting in a uni-directional light beam with a millimetersized circular aperture and 3 divergence. The laser coherence, considered undesirable for imaging purposes, is reduced while maintaining a nanoseconds pulse duration. The properties of the illumination by laser-induced fluorescence (iLIF) are quantified, and a comparison is made with other high-intensity pulsed and continuous light sources.